Analysis Note

ControlHEK-293 cell lysate.

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Anti-PI3 Kinase Antibody, p110β is an antibody against PI3 Kinase for use in WB, IP, IC, IH, ELISA.

Research CategorySignaling

ELISA:
This antibody was tested and determined to work for ELISA at 0.5 ng/well.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

Immunogen

Epitope: Internal Domain

KLH-conjugated synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110β.

Linkage

Replaces: 04-399

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Antigen affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl, with 0.05% NaN3.

Quality

Evaluated by Western Blot on HEK-293 cell lysate.
Western Blot Analysis: 0.05 - 0.1 µg/mL of this antibody detected endogenous PI3 Kinase, p110β in lysate from HEK-293 cells.

Specificity

Recognizes the internal domain of PI3 Kinase, p110β.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Target description

110 kDa